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ligand
protein G
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 4 × 500 μL
制造商/商品名称
Cytiva 28-9513-79
储存条件
(20% Ethanol, 20% medium slurry)
参数
Room temperature temp. range
基质
paramagnetic, spherical, highly cross-linked agarose particles
容量
13- 22 mg binding capacity(human IgG/ml medium)
储存温度
2-8°C
一般描述
Protein G Mag Sepharose™ are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation applications. Based on Sepharose™ with Protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies.
应用
Protein G Mag Sepharose™ are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose™ with Protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies from a broad variety of species. There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis. Protein G Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which has excellent properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.
特点和优势
- Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
- Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
- Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Sepharose is a trademark of Cytiva
商品
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
实验方案
Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.
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