产品名称
Protein A Mag Sepharose™, Cytiva 28-9513-78, pack of 4 × 500 μL
ligand
native protein A
packaging
pack of 4 × 500 μL
manufacturer/tradename
Cytiva 28-9513-78
storage condition
(20% Ethanol, 20% medium slurry)
parameter
Room temperature temp. range
matrix
paramagnetic, spherical, highly cross-linked agarose particles
capacity
8- 17 mg binding capacity(human IgG/ml gel)
storage temp.
2-8°C
相关类别
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Application
Protein A Mag Sepharose™ are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose™ with native Protein A as ligand. Protein A has high affinity for the Fc region of IgG antibodies from a broad variety of species. There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis. Protein A Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.
Features and Benefits
- Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
- Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
- Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis
General description
protein A Mag Sepharose™ are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose™ with native protein A as ligand.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
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