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ligand
phenyl
表单
prepacked column
交联
cross-linked
包装
pkg of 4.7 mL
制造商/商品名称
Cytiva 28-9505-16
储存条件
(20% Ethanol)
参数
44 psi
技术
liquid chromatography (LC): suitable
柱床尺寸
7.7 mm × 100 mm
柱床体积
4.7 mL
色谱柱内径
7.7 mm
基质
6% cross-linked agarose
基质活性基团
Phenyl functional group
粒径
24-44 μm
平均零件尺寸
34 μm
cleaning
2-14
工作范围
3-13
适用性
suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium
分离技术
hydrophobic interaction (HIC)
一般描述
HiScreen™ Phenyl HP columns are prepacked with Phenyl Sepharose™ High Performance hydrophobic interaction chromatography (HIC) media. The columns are an excellent choice for method optimization and parameter screening.
应用
The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ HIC columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.
特点和优势
- Butyl Sepharose™ High Performance is an aliphatic HIC medium, designed for intermediate and polishing step purification steps when high resolution has priority.
- Prepacked, ready-to-use columns for convenient process development optimization.
- 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
- Easily connected in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 0.01 M NaOH)
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
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