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ligand
quaternary amine
描述
Ion Exchanger Type (value)
包装
pkg of 1 ea
制造商/商品名称
Cytiva 28-9505-10
参数
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
柱床尺寸
7.7 mm × 100 mm
柱床体积
4.7 mL
色谱柱内径
7.7 mm
基质
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14
工作范围
2-12
适用性
suitable for bioprocess medium
一般描述
HiScreen™ Q FF columns are prepacked with Q Sepharose™ Fast Flow, a strong anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.
应用
The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.
特点和优势
- Q Sepharose™ Fast FLow is a strong anion exchanger and IEX Sepharose™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
- Prepacked, ready-to-use columns for convenient process development optimization.
- 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
- Easily connected in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
新产品
商品
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
实验方案
This page discusses how to do a purification with SOURCE™ media from Cytiva.
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