产品名称
HiTrap® Albumin IgG Depletion, Cytiva 28-9466-03, pack of 2 × 1 mL
ligand
recombinant protein G fragment and recombinant protein binding HSA
feature
wetted part: no
packaging
pack of 2 × 1 mL
manufacturer/tradename
Cytiva 28-9466-03
storage condition
(20% Ethanol)
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
avg. part. size
34 μm
cleaning
2-9
working range
3-9
storage temp.
2-8°C
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Application
HiTrap® Albumin & IgG Depletion and Albumin & IgG Depletion SpinTrap™ are prepacked columns for the depletion of albumin and IgG from plasma and serum samples. Albumin and IgG are the most abundant plasma Proteins, and they tend to obscure the signals of less abundant Proteins. Depletion of the two Proteins removes more than 60% of the total Protein content in human plasma, allowing a higher load of less abundant Proteins to be included in the analysis and detection of more Proteins. These columns are members of the Trap platform and address the need for flexible, small-scale preparation of Protein samples prior to downstream analyses such as 1-D or 2-D gel electrophoresis and mass spectrometry.
Features and Benefits
- HiTrap® Albumin & IgG Depletion is a prepacked column for the depletion of albumin and IgG from human serum or plasma.
- HiTrap® Albumin & IgG Depletion column provides rapid and easy processing in a convenient format for handling larger sample volumes (~150 μL) using a liquid chromatography system or manually using a syringe.
- Columns are prepacked with high performance Sepharose™ based media with an affinity for human serum albumin (HSA) and IgG.
- High depletion capacity, removes > 95% HSA and > 90% IgG, with high reproducibility.
- Simple and fast procedure.
- Removal of albumin and IgG allows a higher load of less abundant Proteins to be included in the analysis and detection of more Proteins.
General description
HiTrap® Albumin & IgG Depletion is prepacked columns for the depletion of albumin and IgG from human serum and plasma. The columns are prepacked with high performance SepharoseTM based media with an affinity for human serum albumin (HSA) and IgG.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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实验方案
This page shows how to purify or remove albumin and IgG with Albumin & IgG Depletion Sepharose High Performance from Cytiva.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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