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ligand
native protein A
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 1 × 500 μL
制造商/商品名称
Cytiva 28-9440-06
储存条件
(20% Ethanol, 20% medium slurry)
参数
Room temperature temp. range
基质
paramagnetic, spherical, highly cross-linked agarose particles
容量
8- 17 mg binding capacity(human IgG/ml gel)
储存温度
2-8°C
相关类别
一般描述
应用
There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis.
Protein A Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.
特点和优势
- Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
- Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
- Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis
分析说明
法律信息
商品
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
实验方案
Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.
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