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物料
polypropylene (PP)
分子量
40000-20000000 Da (globular proteins)
包装
pkg of 1 ea
制造商/商品名称
Cytiva 28-9356-06
参数
<0.5 mL/min flow rate
<5 mL sample volume
0.15 Mpa (22 psi)
柱床体积
120 mL
色谱柱长度 × 内径
60 cm × 16 mm
基质
dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric
基质活性基团
dextran-acrylamide phase
平均零件尺寸
50 μm
cleaning
2-13
工作范围
3-11
分离技术
size exclusion (SEC)
一般描述
HiPrep™ 26/60 Sephacryl® S-500 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl® S-500 is used for separating polysaccarides, macromolecules with extended structures, and even small plasmids.
特点和优势
- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl Dextran/bisacrylamide matrix.
- Gel filtration columns for Low to medium pressure ÄKTA® systems.
- Well-established in industrial production.
- The HiPrep™ Sephacryl® series offers reproducible separations over a wide molecular weight range.
- Convenient, prepacked HiPrep™ Sephacryl® columns speed up Protein purification.
适用性
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
gel filtration columns for low to medium pressure ÄKTA systems+AE14
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
新产品
实验方案
本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离，该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。
Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.
相关内容
Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.
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