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安全信息

GE28-9321-71

HIS Spintrap™ Kit

Cytiva 28-9321-71, pack of 1 kit

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

包装

pack of 1 kit

制造商/商品名称

Cytiva 28-9321-71

储存条件

(0.15% Kathon CG)
 (20% Ethanol)

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning

2-14(Ni2+-stripped medium.)

工作范围

3-12(Ni2+-stripped medium.)

容量

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

储存温度

2-8°C

相关类别

一般描述

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

特点和优势

  • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
  • Ni Sepharose High Performance in prepacked spin columns.
  • Yields up to 750 µg pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

组分

The kit contains:

1 pack - GE28-4013-53 - His SpinTrap™
1 pack - GE11-0034-00 - His Buffer Kit

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

SpinTrap is a trademark of Cytiva

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • GE11-0034-00His Buffer Kit, Cytiva 11-0034-00化学品安全说明书
  • GE28-4013-53His Spintrap™, Cytiva 28-4013-53化学品安全说明书

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H314

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

法规信息

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商品

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

实验方案

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

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