ligand
alkali-stabilized protein A-derived (E. coli)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9269-77
storage condition
(20% Ethanol)
parameter
44 psi
bed size
7.7 mm × 100 mm
bed volume
4.7 mL
column I.D.
7.7 mm
matrix
highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-13.7
working range
3-12
capacity
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiScreen™ MabSelect™ SuRe™ is prepacked with MabSelect™ SuRe™ and is part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies (MAbs).
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Application
Lab Purification using the MabSelect™ SuRe™ Protein A tag for capture of antibodies.
Features and Benefits
- The novel, alkali-tolerant recombinant protein A ligand of MabSelect™ Sure allows cleaning-in-place (CIP).
- Prepacked, ready-to-use columns for convenient process development.
- 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
HiScreen is a trademark of Cytiva
MabSelect is a trademark of Cytiva
SuRe is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
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