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GE28-9187-79

MBPTrap™ HP

Cytiva 28-9187-79, pack of 1 × 5 mL

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About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

dextrin

包装

pack of 1 × 5 mL

制造商/商品名称

Cytiva 28-9187-79

储存条件

(20% Ehtanol)

参数

<20 mL/min flow rate
42 psi

柱床尺寸

16 mm × 25 mm

柱床体积

5 mL

色谱柱内径

16 mm

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning

2-13

工作范围

>7

容量

~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)

储存温度

2-8°C

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一般描述

MBPTrap™ HP columns prepacked with Dextrin Sepharose™ High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP). This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.

Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

特点和优势

  • HiTrap™ columns prepacked with Dextrin Sepharose™ High Performance medium.
  • Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
  • Physiological conditions and mild elution preserve the activity of the target protein.
  • Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

MBPTrap is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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分析证书(COA)

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Anh Hoang Le et al.
The Journal of cell biology, 220(9) (2021-06-25)
The Scar/WAVE complex drives actin nucleation during cell migration. Interestingly, the same complex is important in forming membrane ruffles during macropinocytosis, a process mediating nutrient uptake and membrane receptor trafficking. Mammalian CYRI-B is a recently described negative regulator of the

商品

Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins

Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.

Characteristics of Dextrin Sepharose® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

实验方案

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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