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包装
pack of 1 × 25 pieces
制造商/商品名称
Cytiva 28-9042-67
一般描述
PlasmidPrep Midi Flow Kit is a gravity flow plasmid prep kit designed for the purification of large yields of transfection-grade plasmid DNA that is proportional to the input sample volume.
The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell′s nucleus, essential for subsequent protein expression of the gene of interest.
PlasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 mL for high copy number plasmid DNA and up to 500 mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.
PlasmidPrep Midi Flow Kit contains 250 fast-flow plasmid columns, resuspension, lysis, neutralization, wash, elution, and Tris-EDTA (TE) buffers, and a full protocol booklet with a detachable, quick reference protocol card.
The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell′s nucleus, essential for subsequent protein expression of the gene of interest.
PlasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 mL for high copy number plasmid DNA and up to 500 mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.
PlasmidPrep Midi Flow Kit contains 250 fast-flow plasmid columns, resuspension, lysis, neutralization, wash, elution, and Tris-EDTA (TE) buffers, and a full protocol booklet with a detachable, quick reference protocol card.
特点和优势
- Provides plasmid DNA with excellent purity, > 70% (strain-dependent) of supercoiled plasmid DNA
- Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns
- Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns.
- Consistently produces pure plasmid DNA with low levels of endotoxin contamination, < 10 endotoxin units (EU) per μg of plasmid DNA
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
illustra is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
新产品
商品
This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.
This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.
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