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ligand
native protein A
物料
polypropylene and polyethylene
特点
wetted part: no
包装
pkg of 4 ea
制造商/商品名称
Cytiva 28-9031-33
储存条件
(20% Ethanol)
参数
30 °C max. temp.
4 °C min. temp.
尺寸
127.8 mm × 85.5 mm × 30.6 mm
基质
highly cross-linked 6% agarose
平均零件尺寸
34 μm
cleaning
2-10
工作范围
3-9
储存温度
2-8°C
一般描述
Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species.
Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
特点和优势
- Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
- Flexible format, with protocols for classic elution and cross-link method.
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Fast binding kinetics and high capacity provides high yield.
- Easy scale-up with HiTrap® Protein A HP prepacked columns.
- Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HiTrap is a registered trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
新产品
商品
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
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