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GE28-4083-47

Ab Spintrap™

Cytiva 28-4083-47

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.25

ligand

recombinant protein G lacking albumin-binding region

包装

pkg of 50 × 100 μL

制造商/商品名称

Cytiva 28-4083-47

储存条件

(20% Ethanol)

柱床体积

100 μL

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning

2-9

工作范围

3-9

容量

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
 >1 mg binding capacity(IgG/column)

储存温度

2-8°C

相关类别

一般描述

Ab SpinTrap™ are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

应用

Ab SpinTrap™ is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.


The spin columns are also suitable for use in antibody screening experiments.


Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.


Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.


For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit.

特点和优势

  • Rapid and convenient small-scale purification of IgG.
  • Protein G Sepharose™ High Performance in prepacked spin columns.
  • No filtration or centrifugation of different crude samples necessary for antibody purification.
  • Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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分析证书(COA)

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商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

实验方案

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

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