推荐产品
ligand
recombinant protein A (E. coli)
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 5 × 5 mL
制造商/商品名称
Cytiva 28-4082-56
储存条件
(20% Ehtanol)
(20% Ethanol)
参数
42 psi
柱床尺寸
16 mm × 25 mm
柱床体积
5 mL
色谱柱内径
16 mm
基质
highly cross-linked agarose
平均零件尺寸
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-12(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-10
容量
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
适用性
suitable for bioprocess medium
储存温度
2-8°C
相关类别
一般描述
应用
特点和优势
- HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
分析说明
其他说明
法律信息
商品
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
本页介绍了高效的色谱柱填充和抗体亲和层析的制备。
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
实验方案
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
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