产品名称
HiTrap® Capto™ Adhere, Cytiva 28-4058-46, pack of 5 × 5 mL
ligand
N-benzyl-N-methyl ethanolamine
description
Ion Exchanger Type (value)
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-4058-46
parameter
<20 mL/min flow rate
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked agarose
avg. part. size
75 (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-14(pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.)
working range
3-12(pH interval where the medium can be operated without significant change in function.)
suitability
suitable for bioprocess medium
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Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Application
Protein polishing using Capto™ Adhere Multimodal chromatography
Features and Benefits
- High capacity and productivity in flowthrough mode.
- Savings in time and operating costs with a two-step chromatographic process.
- Wide operational window of pH and conductivity.
- Prepacked HiTrap columns for easy screening and convenient process development.
General description
HiTrap Capto adhere is prepacked with Capto adhere, a multimodal BioProcess ion exchange resin designed to remove contaminats in post-protein A purification. Capto adhere is a strong anion exchanger with multimodal functionality. The multimodal functionality gives a different selectivity compared to traditional anion exchangers. Capto adhere is designed for post-protein A purification of monoclonal antibodies. Removal of leached protein A, aggregates, host cell proteins, nucleic acids, and viruses from monoclonal antibodies is performed in flowthrough mode at which the antibodies pass directly through the column while the contaminants are adsorbed. For optimal performance of Capto adhere, screening/optimization of loading conditions are required.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Legal Information
Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
新产品
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实验方案
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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