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GE28-4017-47

GSTrap™ 4B

Cytiva 28-4017-47, pack of 1 × 5 mL

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UNSPSC代码:
23151816
NACRES:
NA.56

ligand

glutathione

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包装

pack of 1 × 5 mL

制造商/商品名称

Cytiva 28-4017-47

参数

42 psi (H2O at 25°C)

柱床尺寸

16 mm × 25 mm

柱床体积

5 mL

色谱柱内径

16 mm

基质

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

4-13

working range

4-13

容量

>25 mg binding capacity (horse liver GST/ml medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)

相关类别

一般描述

GSTrap™ 4B columns are prepacked Glutathione Sepharose™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

应用

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System.

特点和优势

  • High binding capacity.
  • Mild elution conditions preserving Protein antigenicity and function.
  • Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
  • GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA® design.

储存及稳定性

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

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Anh Hoang Le et al.
The Journal of cell biology, 220(9) (2021-06-25)
The Scar/WAVE complex drives actin nucleation during cell migration. Interestingly, the same complex is important in forming membrane ruffles during macropinocytosis, a process mediating nutrient uptake and membrane receptor trafficking. Mammalian CYRI-B is a recently described negative regulator of the

商品

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

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实验方案

GSTrap™ Columns for Purification

GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

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