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保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
制造商/商品名称
Cytiva 28-4013-51
包装
kit of one pack of His buffer, pack of 2 × 10 columns
储存条件
(20% Ethanol)
柱床体积
1 mL
容量
40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)
储存温度
2-8°C
一般描述
His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.
The ready to use column is prepacked with Ni Sepharose 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.
His GraviTrap columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.
The His GraviTrap can also be separately purchased:.
分析说明
法律信息
实验方案
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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