跳转至内容
Merck
CN
所有图片(1)

主要文件

安全信息

GE28-4009-90

His MultiTrap™ Fast Flow

Cytiva 28-4009-90, pack of 4 plates

别名:

His Tag Affinity Purification

登录查看公司和协议定价
所有图片(1)

About This Item

UNSPSC代码:
12161501
NACRES:
NA.32

物料

polypropylene and polyethylene

包装

pack of 4 plates

制造商/商品名称

Cytiva 28-4009-90

尺寸

127.8 mm × 85.5 mm × 30.6 mm

基质

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14(Ni2+-stripped medium.)

工作范围

3-12(Ni2+-stripped medium.)

容量

~40 mg binding capacity(histidine-tagged protein)

适用性

suitable for bioprocess medium

一般描述

His MultiTrap™ FF and His MultiTrap™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).

应用

His MultiTrap™ FF and His MultiTrap™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose™ 6 Fast FLow (His MultiTrap™ FF) or Ni Sepharose™ High Performance (His MultiTrap™ HP). A protocol for His MultiTrap™ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.

Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.

特点和优势

  • Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
  • Increased convenience and consistency with prepacked 96-well plates.
  • No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
  • Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
  • Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形图

Environment
GHS09

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

抱歉，我们目前尚未线上提供该产品的COA。

如需帮助，请联系 客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

商品

Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

Performing a Purification and On-column Refolding of an Insoluble Histidine-tagged Protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

查看所有结果

实验方案

Performing a Small-Scale Expression Screening of Histidine-Tagged Membrane Proteins from E. coli Lysates

This page shows how to perform a small-scale expression screening of histidine-tagged membrane proteins from E. coli lysates with His MultiTrap filter plates from Cytiva.

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using Cytiva products.

Ni Sepharose 6 Fast Flow for Protein Purification

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门