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物料
polypropylene and polyethylene
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 4 plates
制造商/商品名称
Cytiva 28-4009-90
尺寸
127.8 mm × 85.5 mm × 30.6 mm
基质
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
容量
~40 mg binding capacity(histidine-tagged protein)
适用性
suitable for bioprocess medium
一般描述
应用
Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.
特点和优势
- Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
- Increased convenience and consistency with prepacked 96-well plates.
- No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
- Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
- Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.
储存及稳定性
分析说明
法律信息
商品
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
实验方案
This page shows how to perform a small-scale expression screening of histidine-tagged membrane proteins from E. coli lysates with His MultiTrap filter plates from Cytiva.
This page shows how to purify protein A-tagged proteins using Cytiva products.
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
How to isolate proteins from inclusion bodies using Cytiva products.
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