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GE27-5330-02

Microspin™ G-50 Columns

Cytiva 27-5330-02, pack of 250 columns

别名:

Microspin columns

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About This Item

UNSPSC代码:
41105501
NACRES:
NA.52

包装

pack of 250 columns

制造商/商品名称

Cytiva 27-5330-02

粒径

50-150 μm (dry)

相关类别

一般描述

MicroSpin™ G-50 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 12-50 ul. They are suitable for any DNA greater than 20 bases in length. They will not remove or denature enzyme.

特点和优势

  • For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μ using spin-column chromatography.
  • Columns are prepacked with Sephadex® G-50 Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide.
  • Product recovery will be at least 10% lower than that from ProbeQuant™ G-50 Micro Columns.
  • Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 μ) following heat denaturation and phenol extraction.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

MicroSpin is a trademark of Cytiva
ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

法规信息

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Domagoj Baretić et al.
Molecular cell, 78(5), 926-940 (2020-05-06)
The eukaryotic replisome, organized around the Cdc45-MCM-GINS (CMG) helicase, orchestrates chromosome replication. Multiple factors associate directly with CMG, including Ctf4 and the heterotrimeric fork protection complex (Csm3/Tof1 and Mrc1), which has important roles including aiding normal replication rates and stabilizing

实验方案

DNA Cleanup using illustra™ GFX Kits

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