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保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
制造商/商品名称
Cytiva 27-5325-01
包装
pkg of 50 columns
粒径
20-80 μm (dry)
working range
2-13
相关类别
一般描述
MicroSpin™ G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.
特点和优势
- For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
- Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
- Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
- Ready to use: Requires less than 4 min from sample application to collection of purified product.
- Tested in nickase, single and double-stranded exonuclease and RNase assays.
- Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.
储存及稳定性
Store at 20 to 25 °C. (Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
法规信息
新产品
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This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
DNA cleanup using illustra™ GFX kits from Cytiva.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。
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