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用途
sufficient for 50 reactions
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
制造商/商品名称
Cytiva 27-0361-01
相关类别
一般描述
Ready-To-Go™ T4 DNA Ligase. Catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.
应用
Applications include self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA. Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 μL, yields a solution of reaction buffer, T4 DNA ligase, and ATP.
特点和优势
- For covalently-linking DNA fragments in as little as 30 min.
- Single-dose, ambient-temperature-stable reaction mixes.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Ready-To-Go is a trademark of Cytiva
法规信息
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