用途
sufficient for 100 reactions
制造商/商品名称
Cytiva 25-6600-31
运输
dry ice
储存温度
−70°C
相关类别
一般描述
Illustra GenomiPhi™ V2 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 4 to 7 μg yield from nanogram amounts of DNA sample.
illustra™ GenomiPhi™ V2 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for mini-scale whole genome amplification by isothermal strand displacement amplification.
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.
The illustra™ GenomiPhi™ V2 DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 4 to 7 μg DNA can be achieved in less than two hours with little hands-on time. The average product length is over 10 kb. The starting material for GenomiPhi™ reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. The kit was verified with DNA from various clinical samples including FTA™ card lysates, blood and buccal swabs.
GenomiPhi™ amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.
illustra™ GenomiPhi™ V2 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for mini-scale whole genome amplification by isothermal strand displacement amplification.
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.
The illustra™ GenomiPhi™ V2 DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 4 to 7 μg DNA can be achieved in less than two hours with little hands-on time. The average product length is over 10 kb. The starting material for GenomiPhi™ reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. The kit was verified with DNA from various clinical samples including FTA™ card lysates, blood and buccal swabs.
GenomiPhi™ amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.
应用
Application For mini-scale whole genome amplification by isothermal strand displacement amplification; typical downstream applications are are genotyping (SNP, STR, array CGH) and DNA archiving.
特点和优势
- Representative isothermal amplification of the whole genome.
- No template-independent, background amplification product.
- Outperforms PCR-based whole genome amplification techniques.
- Quick and simple automation-friendly protocol; no thermal cycler required.
- Yields high-quality DNA (e.g., for high-throughput genotyping, hybridization, and DNA archival).
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
S-re the kit at -70°C. The enzyme mix must be s-red at -70°C; all other components may be s-red at -20°C Thaw components on ice and maintain at 0°C - 4°C during handling
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
GenomiPhi
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.
FTA is a trademark of Qiagen Group
GenomiPhi is a trademark of Cytiva
illustra is a trademark of Cytiva
储存分类代码
12 - Non Combustible Liquids
法规信息
新产品
实验方案
GenomiPhi™ method from Cytiva offers isothermal DNA amplification without a thermal cycler, using Phi29 DNA polymerase.
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