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形式
liquid
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
特点
wetted part: no
包装
pkg of 1 mL
制造商/商品名称
Cytiva 17-6003-18
储存条件
(Rehydration Solution -20)
颜色
Colorless
储存温度
2-8°C
相关类别
一般描述
DeStreak™ Reagent.
应用
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.
DeStreak™ Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak™ Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.
DeStreak™ Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak™ Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak™ Reagent is also available separately in a 1-ml pack size.
DeStreak™ Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak™ Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.
DeStreak™ Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak™ Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak™ Reagent is also available separately in a 1-ml pack size.
特点和优势
- Improves reproducibility and quality of 2-D gels by preventing streaking.
- Eliminates extra spots caused by nonspecific oxidation of Proteins.
- Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
- Highly suitable for both analytical and preparative gels.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
DeStreak is a trademark of Cytiva
警示用语：
Danger
危险声明
危险分类
Acute Tox. 3 Oral - Eye Dam. 1
WGK
WGK 3
闪点(°F)
233.6 °F
闪点(°C)
112 °C
法规信息
新产品
商品
This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.
实验方案
This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
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