HiTrap^® IEX Selection Kit

HiTrap^® IEX Selection Kit consists of seven HiTrap^® columns, prepacked with different Sepharose^™ Fast Flow ion exchange chromatography media for screening of the most appropriate media and conditions to use for specific application and development work.

Q, SP, DEAE, and CM Sepharose^™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose^™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose^™ XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose^™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose^™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP))

Store at 4 to 30 °C (20% Ethanol)

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva