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ligand
recombinant protein L (E. coli), mammalian free
包装
pack of 5 × 1 mL
制造商/商品名称
Cytiva 17-5478-51
储存条件
(20% Ehtanol)
参数
5 bar
色谱柱内径
7 mm
基质
rigid, highly cross-linked agarose
平均零件尺寸
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
工作范围
2-10(pH interval where the medium can be operated without significant change in function.)
适用性
suitable for bioprocess medium
储存温度
2-8°C
一般描述
HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
HiTrap® Protein L columns are packed with Capto™ L affinity chromatography resin. It facilitates the purification of antibody fragments comprising the κ light chain. Capto™ L resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which binds to the variable region of antibody κ light chains with strong affinity. This resin is an effective capture platform for antibody fragments having κ light chains due to its strong binding capacity and affinity to a wide spectrum of antibody fragments.
应用
HiTrap® Protein L may be used to characterize human colorectal cancer MDR1/P-gp Fab and for the purification optimization of a single-chain variable fragment (scFv) against type 1 insulin-like growth factor receptor (IGF-1R). It may also be used for the purification of antigen-binding fragments (Fabs) and domain antibodies (dAbs).
特点和优势
- Captures a wide range of antibody fragments
- Allows a platform approach for the purification of antibody fragment
- Offers low ligand leakage, high binding capacity, and selectivity for a broad range of antibody fragments
- Reduces process time and amount of resin
- Suitable for process development and small-scale purification
- HiTrap® format easily connects to a syringe, peristaltic pump, or chromatographic equipment, such as an ÄKTA® system.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
新产品
实验方案
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
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