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ligand
alkali-stabilized protein A-derived (E. coli)
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 200 mL
制造商/商品名称
Cytiva 17-5474-02
储存条件
(20% Ehtanol)
基质
highly cross-linked agarose
平均零件尺寸
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning in place
2-13.7(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-12(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
容量
~60 mg binding capacity(human IgG/ml medium. Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 100 cm/h in a column with a bed height of 20 cm, i.e. residence time is 6.0 min.)
适用性
suitable for bioprocess medium
储存温度
2-8°C
相关类别
一般描述
MabSelect™ SuRe™ LX is a member of the MabSelect™ family of affinity chromatography media for the capture of monoclonal antibodies (MAbs) at process scale. MabSelect™ SuRe™ LX shares features and benefits of MabSelect™ and MabSelect™ SuRe™ such as a rigid, high-flow agarose matrix that ensures excellent pressure/flow properties, low non-specific binding that leads to low impurity levels in the eluate pool, and an oriented ligand coupling for optimal binding capacity. It also features the same alkali-tolerant rProtein A ligand as MabSelect™ SuRe. This ligand provides greater stability than conventional protein A-based media in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols. The enhanced alkali stability of MabSelect™ SuRe™ improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl. Process economy is also improved by the increased dynamic binding capacity of MabSelect™ SuRe™ LX.
Just as for MabSelect™ SuRe, the novel ligand construct of MabSelect™ SuRe™ LX has been proven to have an increased stability towards proteases. Therefore, the level of leakage of the MabSelect™ SuRe™ LX ligand during elution is low.
MabSelect™ SuRe™ has been demonstrated to give generic elution conditions for different monoclonal antibodies which can be applied also to MabSelect™ SURe LX since it utilizes the same ligand. This is an advantage when designing generic purification platform processes. The feature is also correlated to the construction of the MabSelect™ SuRe™ ligand.
MabSelect™ SuRe™ LX is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect™ SuRe™ meets industrial demands with security of supply and comprehensive technical and regulatory support.
特点和优势
- Novel, alkali-tolerant rProtein A ligand withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
- Reduces the risk of product contamination and carryover
- Improves performance and reduces overall costs
- High dynamic binding capacity (DBC) for high titer cultures reduces process time and amount of medium used
- Generic elution profile for different monoclonal antibodies enables platform approach to purification
- High-flow agarose matrix allows the processing of large feed volumes
分析说明
法律信息
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
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