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ligand
native protein A (S. aureus)
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 5 mL
制造商/商品名称
Cytiva 17-5280-01
储存条件
(20% Ehtanol)
基质
4% cross-linked agarose
平均直径
90 μm (d50v)
cleaning
2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-9
容量
>30 mg binding capacity(human IgG/ml)
适用性
suitable for bioprocess medium
储存温度
2-8°C
相关类别
一般描述
nProtein A Sepharose™ 4 Fast Flow is native protein A coupled to the well established Sepharose™ 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose™ 4 Fast Flow is manufactured without using animal-derived components.
nProtein A Sepharose™ 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose™ CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose™ 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.
As member of the BioProcess media range, nProtein A Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特点和优势
- Replaces Protein A Sepharose™ 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
- Used in routine commercial production of monoclonal antibodies
- Free from animal-derived components.
分析说明
法律信息
商品
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
实验方案
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。
相关内容
利用亲和力或GST沉降、串联亲和纯化（TAP）和免疫共沉淀法研究体外蛋白-蛋白相互作用所需的pull-down检测方法、试剂和实验方案。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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