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GE17-5268-02

Ni Sepharose™ High Performance

Cytiva 17-5268-02, pack of 100 mL

别名:

High Performance Ni Resin, Ni Sepharose

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

包装

pack of 100 mL

制造商/商品名称

Cytiva 17-5268-02

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning in place

2-14(Ni2+-stripped medium.)

工作范围

3-12(Ni²⁺-stripped medium.)

容量

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

相关类别

一般描述

Ni Sepharose™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

特点和优势

  • High-performance purification of histidine-tagged proteins
  • Negligible leakage of Ni2+ ions.
  • High binding capacity, at least 40 mg/mL medium
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • 34 μm bead size for improved resolution

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

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Environment
GHS09

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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How to optimize purification of histidine-tagged proteins using Cytiva products.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

实验方案

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Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

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