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Merck
CN

GE17-5268-01

Ni Sepharose™ High Performance

Cytiva 17-5268-01, pack of 25 mL

别名:

FPLC column packing, HIS-tagged affinity substrate, IMAC resin, histidine affinity polymer, immobilized metal affinity chromatography matrix, stationary phase

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NACRES:
NA.56
UNSPSC Code:
41106500

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产品名称

Ni Sepharose™ High Performance, Cytiva 17-5268-01, pack of 25 mL

crosslinking

cross-linked

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-5268-01

storage condition

(20% ethanol)

parameter

150 cm/hr max. flow rate

technique(s)

liquid chromatography (LC): suitable

matrix

highly cross-linked 6% agarose

matrix active group

Nickel

particle size

~34 μm

cleaning in place

2-14(Ni2+ stripped medium.)

operating pH

3-12(Ni²⁺ stripped medium.)

capacity

≥40 mg binding capacity (histidine-tagged protein / ml medium)

separation technique

affinity

storage temp.

2-30°C

相关类别

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Legal Information

Sepharose is a trademark of Cytiva
IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • High-performance purification of histidine-tagged proteins
  • Negligible leakage of Ni2+ ions.
  • High binding capacity, at least 40 mg/mL medium
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • 34 μm bead size for improved resolution

General description

Ni Sepharose™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

pictograms

Environment
GHS09

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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商品

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

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