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安全和法规

GE17-5255-01

HisTrap^™ Fast Flow

Name: HisTrap<SUP>™</SUP> Fast Flow
Brand: SIGMA

Cytiva 17-5255-01, pack of 5 × 5 mL

别名:

HisTrap FF, His-tagged protein purification column, ready-to-use column

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About This Item

UNSPSC代码:

23151816

NACRES:

NA.56

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包装

pack of 5 × 5 mL

制造商/商品名称

Cytiva 17-5255-01

参数

<4 mL/min flow rate
42 psi

柱床尺寸

7 mm × 25 mm

柱床体积

1 mL

色谱柱内径

7 mm

基质

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14(Ni²⁺-stripped medium.)

工作范围

3-12(Ni²⁺-stripped medium.)

容量

~40 mg binding capacity(histidine-tagged protein)

适用性

suitable for bioprocess medium

一般描述

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose^™ 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

特点和优势

High binding capacity, approx. 40 mg/mL medium.
Negligible leakage of Ni²⁺.
Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.