产品名称
HisTrap™ High Performance, Cytiva 17-5247-01, pack of 5 × 1 mL
form
prepacked column
crosslinking
cross-linked
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5247-01
storage condition
(20% Ethanol)
parameter
1 mL/min flow rate (recommended)
5 bar max. pressure
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
matrix
highly cross-linked 6% agarose
matrix active group
Ni2+
avg. part. size
34 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
≥40 mg binding capacity (histidine-tagged protein / ml medium)
separation technique
affinity
shipped in
ambient
storage temp.
2-30°C
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Analysis Note
Features and Benefits
- High binding capacity, at least 40 mg/mL chromatography medium.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible Ni2+ leakage
- Simple manual operation with a syringe, pump, or chromatography system such as AKTA design
General description
HisTrap™ HP 1 mL and 5 mL columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose™ High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.
HisTrap™ HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be copurified with the tagged target protein.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
For convenient scaling up of histidine-tagged protein purification, use 20 mL HisPrep™ FF 16/10 columns. Ni Sepharose™ 6 Fast Flow, the medium prepacked in HisTrap™ FF and HisPrep™ FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
实验方案
This page shows how to purify protein A-tagged proteins using Cytiva products.
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