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安全信息

GE17-5181-01

Sigma-Aldrich

SOURCE 15Q 4.6/100 PE

别名:

SOURCE 15Q Column

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About This Item

UNSPSC代码:
41115700
NACRES:
SB.52

ligand

quaternary ammonium

物料

polypropylene column

描述

Ion Exchanger Type (value)

包装

pkg of 1 ea

参数

580 psi

柱床尺寸

4.6 mm × 100 mm

柱床体积

1.7 mL

色谱柱内径

4.6 mm

基质

polymeric
polystyrene/divinylbenzene

基质活性基团

ammonium ion, quaternary phase

平均零件尺寸

15 μm

cleaning

1-14

工作范围

2-12

适用性

suitable for bioprocess medium

分离技术

anion exchange

一般描述

SOURCE 15Q 4.6/100 PE is a strong anion exchanger prepacked in a Tricorn column for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.

特点和优势

  • SOURCE 15Q media is highly suitable for fast, high-resolution purifications and easy scale-up
  • Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
  • Improved capacity compared to MonoBeads but with a slightly lower resolution.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

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法律信息

SOURCE is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

实验方案

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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