material
borosilicate glass column, polypropylene filter
mol wt
1000-300000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 17-5173-01
parameter
0.5 ml/min - 1 ml/min flow rate, 25-500 mL sample volume, 3 Mpa (435 psi)
technique(s)
HPLC: suitable
bed volume
24 mL
column L × I.D.
30 cm × 10 mm
matrix
highly cross-linked agarose
matrix active group
agarose phase
avg. part. size
11 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
General description
Superose® 12 10/300 GL are prepacked Tricorn columns for-high performance gel filtration with a broad fractionation range of molecules with molecular weights between 1000 and 300 000.
Application
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
for high-resolution separations across exceptionally broad molecular-weight ranges
Features and Benefits
- Achieve high-resolution separations across very broad molecular-weight ranges.
- Low ionic strength eluents (<0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose® for some lipids, peptides, and small aromatic compounds.
- Prepacked Tricorn columns are durable, high-performance glass columns for reliable and reproducible separations.
- Easy, efficient scale-up from prepacked Superose® 10/300 GL columns to Superose® prep grade lab packs.
Preparation Note
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Superose is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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