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ligand
sulphopropyl
描述
Ion Exchanger Type (value)
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 5 × 5 mL
制造商/商品名称
Cytiva 17-5157-01
参数
flow rate
42 psi
柱床尺寸
16 mm × 25 mm
柱床体积
5 mL
色谱柱内径
16 mm
基质
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
3-14
working range
4-13
适用性
suitable for bioprocess medium
相关类别
一般描述
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
特点和优势
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High flow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
储存及稳定性
分析说明
其他说明
法律信息
商品
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
实验方案
Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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