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GE17-5143-01

HiTrap® Benzamidine Fast Flow

Cytiva 17-5143-01, pack of 5 × 1 mL

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About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

p-aminobenzamidine

包装

pack of 5 × 1 mL

制造商/商品名称

Cytiva 17-5143-01

储存条件

(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)

参数

42 psi

柱床尺寸

7 mm × 25 mm

柱床体积

1 mL

色谱柱内径

7 mm

基质

highly cross-linked 4% agarose

平均直径

90 μm

cleaning

1-9

工作范围

2-8

储存温度

2-8°C

一般描述

HiTrap™ Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose™ 4 Fast FLow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.

应用

HiTrap™ Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.

The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.

特点和优势

  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
  • Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
  • HiTrap™ Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
  • High binding capacity.
  • The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA™ design or FPLC™ System.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

HiTrap is a registered trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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商品

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

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实验方案

HiTrap® Benzamidine FF for Thrombin & Factor Xa Removal

Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.

Separation with Cytiva Products (GST Fusion Proteins)

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

亲和层析缓冲液置换和脱盐

如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。

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