ligand
recombinant protein A (E. coli)
packaging
pack of 5 mL
manufacturer/tradename
Cytiva 17-5138-01
matrix
4% cross-linked agarose
particle size
60-165 μm
average diameter
90 μm (d50v)
cleaning in place
2-11
working range
3-10
capacity
>35 mg binding capacity(human IgG/ml)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
storage condition
(20% Ethanol)
General description
rmp protein A Sepharose™ Fast FLow is a low leakage, non-mammalian based affinity medium designed for high purity separation of monoclonal and polyclonal antibodies at the laboratory and process scales.
Application
rmp Sepharose™ 4 Sepharose™ Fast FLow is composed of recombinant Protein A coupled to the well established Sepharose™ 4 Fast FLow base matrix by reductive amination resulting in multipoint attachment.
Reductive amination creates chemically stable amide bonds and resulting Low ligand leakage. rmp Protein A Sepharose™ 4 Fast FLow is manufactured without using animal-derived components. As member of the BioProcess media range, rmp Sepharose™ 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Reductive amination creates chemically stable amide bonds and resulting Low ligand leakage. rmp Protein A Sepharose™ 4 Fast FLow is manufactured without using animal-derived components. As member of the BioProcess media range, rmp Sepharose™ 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Multi-point attachment of rProtein A by chemically-stable amide bonds
- Coupling chemistry that minimizes leakage of Protein A ligand.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
新产品
此项目有
Robert Becker et al.
eLife, 10 (2021-10-05)
Non-centrosomal microtubule-organizing centers (MTOCs) are pivotal for the function of multiple cell types, but the processes initiating their formation are unknown. Here, we find that the transcription factor myogenin is required in murine myoblasts for the localization of MTOC proteins
实验方案
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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