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Merck
CN

GE17-5113-01

Streptavidin Sepharose™ High Performance

Cytiva 17-5113-01, pack of 5 mL

别名:

affinity chromatography medium, high performance affinity resin, biotinylated proteins purification

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NACRES:
NA.56
UNSPSC Code:
41106500

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产品名称

Streptavidin Sepharose™ High Performance, Cytiva 17-5113-01, pack of 5 mL

form

resin

crosslinking

cross-linked

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-5113-01

storage condition

(20% Ethanol)

parameter

4-30 °C temp. range (working)

technique(s)

liquid chromatography (LC): suitable

matrix

highly cross-linked 6% agarose

matrix active group

Streptavidin

avg. part. size

34 μm

working range

2-10.5

capacity

>300 mg binding capacity (Biotin / mL resin)
 6 mg binding capacity (biotinylated BSA / mL resin)

separation technique

affinity

storage temp.

2-8°C

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Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • Streptavidin immobilized to Sepharose™ High Performance for purification of biotinylated compounds
  • Extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
  • 34 μm bead size for improved resolution

General description

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules.

Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilised streptavidin binds biotin and biotinylated substances through affinity chromatography.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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Adar Hacohen et al.
Micromachines, 11(5) (2020-05-21)
The ability to manipulate and selectively position cells into patterns or distinct microenvironments is an important component of many single cell experimental methods and biological engineering applications. Although a variety of particles and cell patterning methods have been demonstrated, most
Jérôme Zervudacki et al.
The EMBO journal, 37(14) (2018-06-07)
Mobilization of transposable elements (TEs) in plants has been recognized as a driving force of evolution and adaptation, in particular by providing genes with regulatory modules that impact their transcription. In this study, we employed an ATCOPIA93 long-terminal repeat (LTR)
Song Zhu et al.
Theranostics, 11(7), 3359-3375 (2021-02-05)
Background: A metabolic "switch" from oxidative phosphorylation to glycolysis provides tumor cells with energy and biosynthetic substrates, thereby promoting tumorigenesis and malignant progression. However, the mechanisms controlling this metabolic switch in tumors is not entirely clear. Methods: Clinical specimens were
Hana Antonicka et al.
Cell metabolism, 32(3), 479-497 (2020-09-03)
We used BioID, a proximity-dependent biotinylation assay with 100 mitochondrial baits from all mitochondrial sub-compartments, to create a high-resolution human mitochondrial proximity interaction network. We identified 1,465 proteins, producing 15,626 unique high-confidence proximity interactions. Of these, 528 proteins were previously
Sarah van Veen et al.
Nature, 578(7795), 419-424 (2020-01-31)
ATP13A2 (PARK9) is a late endolysosomal transporter that is genetically implicated in a spectrum of neurodegenerative disorders, including Kufor-Rakeb syndrome-a parkinsonism with dementia1-and early-onset Parkinson's disease2. ATP13A2 offers protection against genetic and environmental risk factors of Parkinson's disease, whereas loss

商品

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

实验方案

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Performing a Separation of Biotin and Biotinylated Substances with HiTrap Streptavidin HP and Streptavidin Sepharose High Performance

How to separate biotin and biotinylated substances by affinity chromatography using HiTrap Streptavidin HP and Streptavidin Sepharose High Performance products from Cytiva.

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.

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