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ligand
2-mercaptopyridine
包装
pack of 1 × 5 mL
制造商/商品名称
Cytiva 17-5111-01
储存条件
(20% Ethanol)
参数
<20 mL/min flow rate
42 psi
柱床尺寸
16 mm × 25 mm
柱床体积
5 mL
色谱柱内径
16 mm
基质
highly cross-linked 6% agarose
平均零件尺寸
34 μm
cleaning
2-13
工作范围
3-11
储存温度
2-30°C
一般描述
HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose™ High Performance for fast and easy purification of IgY from egg yolk.
应用
HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.
特点和优势
- Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
- Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose™ High Performance.
- Recovery and purity of IgY are excellent.
- Can be operated with a liquid chromatography system such as ÄKTA® design.
- The top and bottom frits are manufactured from porous polyethylene
- Connectors for usage with different chromatography systems and other equipment are included
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他说明
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律信息
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
新产品
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
This page shows how to prepare samples for purification with affinity chromatography.
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