产品名称
HiTrap® IGM purification, Cytiva 17-5110-01, pack of 5 × 1 mL
ligand
2-mercaptopyridine
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5110-01
storage condition
(20% Ethanol)
parameter
<4 mL/min flow rate
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-13
working range
3-11
storage temp.
2-8°C
Analysis Note
Application
Features and Benefits
- Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
- Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
- Recovery of IgM is high with excellent activity retention.
- The top and bottom frits are manufactured from porous polyethylene
- Columns are delivered with a stopper on the inlet and a snap-off end on the outle
General description
Other Notes
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
实验方案
How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.
This page shows how to prepare samples for purification with affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page discusses how to do a purification with SOURCE™ media from Cytiva.
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