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GE17-5110-01

HiTrap® IGM purification

Cytiva 17-5110-01, pack of 5 × 1 mL

别名:

IGM Purification Column

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About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

2-mercaptopyridine

包装

pack of 5 × 1 mL

制造商/商品名称

Cytiva 17-5110-01

储存条件

(20% Ethanol)

参数

<4 mL/min flow rate
42 psi

柱床尺寸

7 mm × 25 mm

柱床体积

1 mL

色谱柱内径

16 mm

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning

2-13

工作范围

3-11

储存温度

2-8°C

一般描述

HiTrap® IgM Purification HP is a 1 mL column prepacked with Sepharose™ High Performancefor fast and efficient purification of monoclonal IgM.

应用

HiTrap® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

特点和优势

  • Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
  • Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
  • Recovery of IgM is high with excellent activity retention.
  • The top and bottom frits are manufactured from porous polyethylene
  • Columns are delivered with a stopper on the inlet and a snap-off end on the outle

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

Performing a Separation with HiTrap® IgM Purifcation HP

How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from Cytiva.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

亲和层析缓冲液置换和脱盐

如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。

查看所有结果

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