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Merck
CN

GE17-5087-01

HiPrep™ 26/10 Desalting

20-80 μm particle size (dry), bed H × diam. 10 cm × 2.6 cm, pkg of 1 ea

别名:

Desalting Column, HiPrep Desalting Column

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NACRES:
SB.52
UNSPSC Code:
41115700

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产品名称

HiPrep™ 26/10 Desalting, 20-80 μm particle size (dry), bed H × diam. 10 cm × 2.6 cm, pkg of 1 ea

form

prepacked column

crosslinking

cross-linked

mol wt

protein Mr 5000 Da (exclusion limit)

packaging

pkg of 1 ea

storage condition

(20% ethanol)

parameter

≤15 mL sample volume
40 mL/min max. flow rate
5 bar max. pressure
9-31 mL/min flow rate

technique(s)

liquid chromatography (LC): suitable

matrix

cross-linked dextran

matrix active group

dextran-epichlorohydrin phase
dextran

cleaning

2-13

working range

2-13

manufacturer/tradename

Cytiva 17-5087-01

bed H × diam.

10 cm × 2.6 cm

bed volume

53 mL

particle size

20-80 μm (dry)

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

相关类别

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps.

Features and Benefits

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

General description

HiPrep™ 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Legal Information

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Automated Antibody Purification with ÄKTA Systems

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

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实验方案

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

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