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ligand
recombinant protein A (E. coli)
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 1 × 5 mL
制造商/商品名称
Cytiva 17-5080-01
储存条件
(20% Ehtanol)
(20% Ethanol)
参数
42 psi
柱床尺寸
16 mm × 25 mm
柱床体积
5 mL
色谱柱内径
16 mm
基质
4% cross-linked agarose
粒径
60-165 μm
平均直径
90 μm (d50v)
cleaning
2-11(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-10
容量
~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
适用性
suitable for bioprocess medium
储存温度
2-8°C
相关类别
一般描述
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
特点和优势
- Quick, convenient preparative purification of monoclonal antibodies.
- Well-established protein A-based medium used in many approved MAb processes.
- Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.
分析说明
其他说明
法律信息
商品
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
实验方案
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
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