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安全信息

GE17-5056-01

HiTrap® CM Fast Flow

Cytiva 17-5056-01, pack of 5 × 1 mL

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About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

carboxymethyl

描述

Ion Exchanger Type (value)

包装

pack of 5 × 1 mL

制造商/商品名称

Cytiva 17-5056-01

参数

42 psi

柱床尺寸

7 mm × 25 mm

柱床体积

1 mL

色谱柱内径

7 mm

基质

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14

工作范围

4-13

适用性

suitable for bioprocess medium

一般描述

HiTrap CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Q, SP, DEAE adn CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

特点和优势

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

HiTrap is a registered trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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商品

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

实验方案

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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