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COO原产地证书/COA分析证书

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安全和法规

GE17-3712-06

HisTrap^™ Excel

Name: HisTrap<SUP>™</SUP> Excel
Brand: SIGMA

Cytiva 17-3712-06, pack of 5 × 5 mL

别名:

affinity resins, Ni Sepharose excel column, ion affinity chromatography column, IMAC column

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About This Item

UNSPSC代码:

23151816

NACRES:

NA.56

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cOmplete^™His标签纯化色谱柱

表单

prepacked column

交联

cross-linked

包装

pack of 5 × 5 mL

制造商/商品名称

Cytiva 17-3712-06

储存条件

(20% Ethanol)

参数

5 bar
5 ml/min - 20 ml/min flow rate

技术

liquid chromatography (LC): suitable

柱床尺寸

16 mm × 25 mm

柱床体积

5 mL

色谱柱内径

16 mm

基质

highly cross-linked 6% agarose

基质活性基团

Nickel

平均直径

90 μm

cleaning

2-14

工作范围

2-13

容量

≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)

分离技术

affinity

一般描述

Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.

HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

应用

Techniques & Applications: Ready-to-use IMAC columns, prepacked with Ni Sepharose^™ excel medium resistant to chelating and reducing agents

特点和优势

Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
Increase target protein yield and decrease degradation through reduced and simplified sample handling
HisTrap excel columns allow direct purification of cell-free, unclarified samples
HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.

The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.