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GE17-1287-10

Anx Sepharose Fast Flow High Sub

Cytiva 17-1287-10, pack of 25 mL

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UNSPSC代码:
47101511
NACRES:
NA.56

ligand

diethylaminopropyl

描述

Ion Exchanger Type (value)

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包装

pack of 25 mL

制造商/商品名称

Cytiva 17-1287-10

基质

4% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

2-14

working range

3-13

适用性

suitable for bioprocess medium

一般描述

ANX Sepharose 4 Fast FLow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers. It is optimized for the separation of larger proteins.

应用

ANX Sepharose Fast FLow (high sub) is part of the Sepharose Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. ANX Sepharose 4 Fast FLow (high sub) is a weak anion exchanger medium with tertiary amine groups attached to the base matrix Sepharose 4 Fast FLow via ether linkage and a hydrophilic spacer arm. The diethylaminopropylgroup is coupled in a mode that precludes formation of quaternary groups, which are typically seen on traditional DEAE media, resulting in a truly weak anion exchanger. The base matrix, Sepharose 4 Fast Flow, is a highly cross-linked, 4% Agarose derivative with high chemical and physical stability and broad separation range. ANX Sepharose 4 Fast FLow (high sub) has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.

As member of the BioProcess media range, ANX Sepharose 4 Fast FLow (high sub) meets industrial demands with security of supply and comprehensive technical and regulatory support.

特点和优势

  • Weak anion exchanger with different selectivity compared with traditional ion exchangers.
  • Applicable for separation of high molecular weight Proteins
  • Developed in co-operation with leading large-scale pharmaceutical manufacturers
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

储存及稳定性

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

法规信息

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商品

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

实验方案

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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