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GE17-1279-03

rProtein A Sepharose™ Fast Flow

Cytiva 17-1279-03, pack of 200 mL

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别名:
antibody affinity medium, antibody purification resin
UNSPSC代码:
41106500
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包装

pack of 200 mL

制造商/商品名称

Cytiva 17-1279-03

储存条件

(20% Ehtanol)

基质

4% cross-linked agarose

粒径

60-165 μm

平均直径

90 μm (d50v)

cleaning in place

2-11

working range

3-10

容量

~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)

适用性

suitable for bioprocess medium

储存温度

2-8°C

相关类别

一般描述

rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium, designed for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

rProtein A Sepharose™ 4 Fast Flow is based on the well established Sepharose™ Fast Flow platform and is used in many approved monoclonal antibody (MAb) processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.

rProtein A Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, rProtein A Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特点和优势

  • High dynamic binding capacity due to oriented, single-point attachment of protein A
  • Well-established Protein A medium used in many approved MAb processes
  • No mammalian components involved during the manufacturing process

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

法规信息

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Maida Romera-Branchat et al.
Cell reports, 31(9), 107717-107717 (2020-06-04)
Flowering of many plant species depends on interactions between basic leucine zipper (bZIP) transcription factors and systemically transported florigen proteins. Members of the genus Arabidopsis contain two of these bZIPs, FD and FDP, which we show have largely complementary expression
Lawrence T Wang et al.
Cell reports, 38(7), 110367-110367 (2022-02-17)
L9 is a potent human monoclonal antibody (mAb) that preferentially binds two adjacent NVDP minor repeats and cross-reacts with NANP major repeats of the Plasmodium falciparum circumsporozoite protein (PfCSP) on malaria-infective sporozoites. Understanding this mAb's ontogeny and mechanisms of binding
Lawrence T Wang et al.
Immunity, 53(4), 733-744 (2020-09-19)
Discovering potent human monoclonal antibodies (mAbs) targeting the Plasmodium falciparum circumsporozoite protein (PfCSP) on sporozoites (SPZ) and elucidating their mechanisms of neutralization will facilitate translation for passive prophylaxis and aid next-generation vaccine development. Here, we isolated a neutralizing human mAb

商品

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

色谱柱填充和抗体亲和层析的制备

本页介绍了高效的色谱柱填充和抗体亲和层析的制备。

实验方案

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

线性流速（cm/小时）与体积流速（mL/min)之间的换算

本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

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