产品名称
rProtein A Sepharose™ Fast Flow, Cytiva 17-1279-01, pack of 5 mL
form
resin
crosslinking
cross-linked
packaging
pack of 5 mL
manufacturer/tradename
Cytiva 17-1279-01
storage condition
(20% Ehtanol)
technique(s)
liquid chromatography (LC): suitable
matrix
4% cross-linked agarose
matrix active group
Recombinant protein A (E. coli)
particle size
~90 μm
cleaning in place
3-12
operating pH
3-10
capacity
~35 mg binding capacity (human lgG / ml medium)
separation technique
affinity
storage temp.
2-8°C
Analysis Note
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
实验方案
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
This page shows how to prepare samples for purification with affinity chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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