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安全信息

GE17-1196-01

Sigma-Aldrich

HiPrep™ 26/60 Sephacryl® S-300 HR

Cytiva 17-1196-01, column L × I.D. 60 cm × 26 mm, 50 μm avg. part. size

别名:

HiPrep Chromatography Column, Sephacryl S-300 HR

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About This Item

UNSPSC代码:
41115700
NACRES:
SB.52

物料

polypropylene (PP)

分子量

10000-1500000 Da (globular proteins)

包装

pkg of 1 ea

制造商/商品名称

Cytiva 17-1196-01

参数

<13 mL sample volume
<2.6 mL/min flow rate
0.15 Mpa (22 psi)

柱床体积

320 mL

色谱柱长度 × 内径

60 cm × 26 mm

基质

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

基质活性基团

dextran-acrylamide phase

平均零件尺寸

50 μm

cleaning

2-13

工作范围

3-11

分离技术

size exclusion (SEC)

一般描述

HiPrep™ 26/60 Sephacryl® S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-300 is used for separating midsize proteins including antibodies and serum proteins.

特点和优势

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep™ Sephacryl® series offer reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.

适用性

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
HiPrep 26/60 Sephacryl S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl S-300 is used for separating midsize proteins including antibodies and serum proteins.
for using preparative separation of biomolecules of up to 13 mL sample volume

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法律信息

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

新产品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number

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实验方案

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from Cytiva.

使用Sephacryl®进行分离

本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离，该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。

Sephacryl® Separation

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

相关内容

Separation Options with Sephacryl®

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

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