ligand
sulfonate group
描述
Ion Exchanger Type (value)
包装
pack of 1 mL
制造商/商品名称
Cytiva 17-1178-01
参数
218 psi
柱床尺寸
6.4 mm × 30 mm
柱床体积
1 mL
基质
polystyrene/divinylbenzene
平均零件尺寸
15 μm
粒径
15-15 μm
cleaning
1-14
working range
2-13
适用性
suitable for bioprocess medium (BioProcess medium. Columns not suitable for use with ÄKTAprime plus system.)
suitable for bioprocess medium
相关类别
一般描述
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
特点和优势
- SOURCE™ 15Q and 15S media are well suited for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
储存及稳定性
分析说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
实验方案
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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