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安全信息

GE17-1167-01

Sigma-Aldrich

HiPrep 16/60 Sephacryl® S-300 HR

Cytiva 17-1167-01, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

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UNSPSC代码:
41115700
NACRES:
SB.52

物料

polypropylene

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分子量

10000-1500000 Da (globular proteins)

包装

pkg of 1 ea

制造商/商品名称

Cytiva 17-1167-01

参数

<1 mL/min flow rate
<5 mL sample volume
0.15 Mpa (22 psi)

柱床体积

120 mL

色谱柱长度 × 内径

60 cm × 16 mm

基质

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

基质活性基团

dextran-acrylamide phase

平均零件尺寸

50 μm

cleaning

2-13

working range

3-11

分离技术

size exclusion (SEC)

一般描述

HiPrep 16/60 Sephacryl® S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-300 is used for separating midsize proteins including antibodies and serum proteins.

特点和优势

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep Sephacryl® series offer reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.

适用性

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 5 mL sample volume

储存及稳定性

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法律信息

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

法规信息

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实验方案

This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from Cytiva.

This page shows how to refold proteins using gel filtration and products from Cytiva.

本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离,该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

相关内容

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

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