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安全信息

GE17-0998-01

Heparin Sepharose 6 Fast Flow

Cytiva 17-0998-01, pack of 50 mL

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UNSPSC代码:
41106500
NACRES:
NA.56

ligand

porcine heparin

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包装

pack of 50 mL

制造商/商品名称

Cytiva 17-0998-01

基质

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

4-13

working range

4-12

适用性

suitable for bioprocess medium

相关类别

一般描述

Heparin Sepharose 6 Fast Flow is a well established BioProcess affinity medium designed for capture or intermediate purification steps. This medium is extensively used for industrial purification of several marketed plasma proteins.

Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand.

This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.

Heparin Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Heparin Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特点和优势

  • Industrial purification of antithrombin III, coagulation factors and other plasma proteins
  • Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand.
  • BioProcess medium supported for industrial applications and well-established in approved processes

储存及稳定性

Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

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商品

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This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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